AMH ELISA Kit is intended for the quantitative measurement of AMH in human serum or plasma.
AMH ELISA is based on solid phase sandwich ELISA method. The samples and conjugate reagent (anti-AMH biotin & HRP) are added to the wells coated with Streptavidin. AMH in the patient's serum binds to the matched pair Abs, forming a sandwich complex and simultaneously the complex is being immobilized on the plate through streptavidin-biotin interactions. Unbound protein and HRP conjugate are washed off, through a washing step. Upon addition of the substrate, the intensity of color is proportional to the concentration of AMH in the samples. A standard curve is prepared by relating the color intensity to the concentration of AMH.
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