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- IRIS11 Prestained Protein Ladder
- Product Detail
- Company Profile
The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Guide for Molecular Weight Estimation (kDa)
Refer to the IRIS11 Prestained Protein Ladder patterns in various electrophoresis conditions:
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
Storage Buffer :
0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
Quality Control :
5μl of IRIS11 Prestained Protein Ladder (PMI11) resolves 11 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
→ More information at http://www.bio-helix.com/products/267.
PMI11-0500_500μl
PMI11-B050ML_50ml
PMI11-B250ML_250ml
PMI11-B500ML_500ml
▍Related Products:
○ PMI09-0500 IRIS9 Plus Prestained Protein Ladder (9 pre-stained bands, 15-170 kDa)
○ PMI11-0500 IRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa)
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Bio-Helix has been focusing on providing the best bio-reagents for life science researchers all over the world since 2007. Our products, to name a few, include DNA markers, protein markers, staining reagents, nucleic acid purification, PCR reagents and so on.
Working together with scientists in the lab around the world and continuing efforts of quality improvement, we begin to gain trusts and appraises from customers. The brand, Bio-Helix, spreading in more than forty countries, has become a trustworthy and welcome brand of reagents in laboratories of life science.
With the expansion of product lines as well as customers, we discover a greater need for a good quality supply of bio-reagents from the medical circle. Mostly, these needs are for diagnostic products. The demand of good quality bio-reagents for diagnostic devices opens a whole new possibility of innovation and motivation for Bio-Helix.
We have a vision that one day our life span can be elongated; life quality can be improved by early detection and subsequent proper treatment of all diseases. Ancient Chinese believed that a good doctor treats the disease before illness, instead of after its onset. In Bio-Helix, a new brand, LifeDirex, is created to fulfill this vision. LifeDirex will be focusing on supplying the best and most innovative reagents for diagnostic tools to promote the earliest detection of diseases as possible.
Our mission is to provide the best quality products and to develop novel technologies that assist researchers to find solutions in quest of scientific achievements. Furthermore, we decide now is the best time to enter the market of in vitro diagnostic device (IVDD). It is our goal to become the best solution provider for both research laboratory and IVDD.
Driven by desires to improve life quality and achieve scientific excellence, we operate with the highest standards of integrity. We also extend the spirit to our services and global value to our customers all around the world. A diverse workforce and network of collaboration from a great mix of company culture. Bio-Helix is not only a company of equal opportunity but also works hard to provide a friendly and growing environment.
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