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- E.coli|360-HCP ELISA kits and Components
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- E.coli|360-HCP ELISA kits and Components
The E.coli|360-HCP ELISA is an enhanced generic host cell protein (HCP) assay. It was designed to cover a broader spectrum of E.coli host cell proteins (HCPs) than other commercially available generic HCP assays.
E.coli|360-HCP ELISA is based on W3110 cell lines which were fermented in different culture media and conditions resulting in several antigen preparations with distinct HCP patterns. By immunizing goats with these antigens, BioGenes has developed a panel of different HCP ELISAs (C and E) that together build up the enhanced generic E.coli|360-HCP ELISA.
High specificity of both antibody preparations (C and E) was demonstrate by coverage of ≥ 80% of the respective E.coli|360-HCP antigen distribution. Antigen coverage was measured by a 2D Western blot with Cy5-labeled E.coli|360-HCP standard and the corresponding Cy3-labeled anti-HCP antibodies.
Satisfying recovery was estimated in spiked samples within the assay working range within the acceptance criterion 100±30% of spiked antigen. High sensitivity of E.coli|360-HCP ELISA kits (C and E), described by the validation parameter LOD, LOQ and working range, is shown below:
•LOD: 0.2 – 0.5 ng/mL
•LOQ: 0.6 – 1.6 ng/mL
•Working range: 2 – 100 ng/mL
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BioGenes is a highly specialized and experienced partner for challenging and customized antibody and immunoassay development projects, with a particular focus on ELISAs for quality control, diagnostics, and the development and testing of new biological therapeutics.
The Berlin-based company, which has been ISO certified since 1999, operates worldwide and maintains long-term business relationships with biotech and pharmaceutical companies, including eight of the world's ten largest pharmaceutical companies. A leading auditing firm has rated the company as one of the world's most important companies in the field of Host Cell Protein (HCP) analysis.
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