1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained.

2. Technical specifications

Sensitivity:  0.5 ppb

Incubator temperature:  25℃

Incubator time:  30min～15min

Detection limit:

Milk powder................................................................................... 10 ppb

Milk................................................................................................. 1 ppb

Feed.............................................................................................. 50 ppb

Cross-reaction rate:

Melamine......................................................................................... 100%

Cyanuric acid..................................................................................... 60%

Triazine.............................................................................................. <1%

Diamino atrazine................................................................................ <1%

Recovery rate:

Milk.............................................................................................. 90±25%

Milk powder.................................................................................. 80±25%

Feed............................................................................................ 80±15%

3. Components

1. Micro-well strips: 12 strips with 8 removable wells each
2. 6× standard solution (1 mL each): 0 ppb,0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb
3. Enzyme conjugate (7 mL)............................................................... red cap
4. Antibody working solution (7 mL).................................................. blue cap
5. Substrate A solution (7 mL)......................................................... white cap
6. Substrate B solution (7 mL)......................................................... black cap
7. Stop solution (7 mL).................................................................. yellow cap
8. 20× concentrated washing buffer (40 mL)..................................... white cap
9. 2× concentrated redissolving solution (50 mL)..................... transparent cap