The test kit allows for the determination of Porcine Toxoplasma antibody (TOX-Ab) expression in swine serum and plasma, can be used to Porcine Toxoplasma vaccine immunization effect assessment
Porcine Toxoplasma IGg Antibody Test Kit
【Specification】
96T/kit 96T*2/kit 96T*5/kit
【Principle of Test】
The kit is for the qualitative determination of Tox ab in the sample, adopt purified Tox Sag1 Recombinant antigen to coat microtiter plate, make solid-phase antigen, then pipette samples to the wells, with anti- Porcine Tox ab conjugated Horseradish Peroxidase (HRP). Wash and remove non-combinative antibody and other components. Antibodies specific for the antigen will bind to the pre-coated antigen. After washing completely, add TMB substrate solution and color develops according to the amount of Tox ab. Reaction is terminated by the addition of a stop solution and the intensity of the color is measured at a wavelength of 450 nm. Compared with the CUTOFF value to judge if Tox ab exists in the sample or not.
【Composition of the Kit】
Microelisa Stripplate、HRP-Conjugate Reagent、Sample Diluent、Concentrated Wash Solution 20×、
Chromogen Solution A、Chromogen Solution B、Stop Solution、Positive control、Negative control
【Storage Conditions】
1. The kit shall be stored at [2-8 ℃].
2. The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
【Instrument】
Mircoplate reader (contain: 450nm, 630nm wavelength), thermostatic equipment (37 degrees Celsius), adjustable Pipette.
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