Abbkine CheKine™ NAD/NADH Assay Kit provides a convenient tool for sensitive detection of the NAD and NADH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NADP+/NADPH), in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NAD+ /NADH concentration in the sample.
Standard Curve of NAD in 96-well plate assay.
The y-axis is ΔOD and the x-axis is NAD concentration (uM).
The Abbkine NAD/NADH Quantification Kit has below features:
1. Detect NAD+/NADH concentrations and ratios in cell or tissue extracts.
2. Detection limit of 0.78 µM and linearity up to 50 µM NAD+/NADH in 96-well plate assay.
More detailed information of Cell Metabolism Assay Kits at here.
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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.