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- SNAP i.d.® 2.0 Protein Detection System for Western Blotting
- Product Detail
- Company Profile
- How to block, probe, wash, detect Western blots in less than
30 minutes
The immunodetection phase of Western blotting, consisting of blocking, antibody incubations, and washing steps, may be subject to experimental variability. The SNAP i.d. 2.0 system both accelerates immunodetection, shortening time to results, and enables parallel processing of blots, making it easy to apply consistent conditions across experiments.
Benefits of SNAP i.d. technology
The SNAP i.d. 2.0 system uses a vacuum manifold to drive blocking reagents, antibodies, and wash buffers directly through the PVDF or nitrocellulose membrane of the blot. As a result, immunodetection is much faster: a process requiring 4-24 hours using traditional, platform shaker-dependent Western blot processing takes only 30 minutes.
Features of the SNAP i.d. 2.0 System
Processing of up to four blots at a time on a vacuum base with two individually controlled sides
Three removable blot holding frame sizes: MultiBlot, Mini, and Midi, to accommodate different blot sizes
Disposable blot holders, sized for MultiBlot, Mini, and Midi frames
Extended and off-line blot processing options: frames have lids and can be removed from the base for extended incubation (one hour to overnight), incubation in a shaker, or incubation at 4 °C
Stackable frames so multiple blots can be processed at the same time
30-minute immunodetection with uniform signal across the blot
Greater than 80% antibody recovery using the Antibody Collection Trays
Compatible with nitrocellulose and polyvinylidene fluoride (PVDF) membranes
Works with the most blocking buffers and visualization methodologies (e.g. chemiluminescence, fluorescence, or colorimetric)
Parts and Functions of the SNAP i.d. 2.0 System
The system consists of the following components:
| SNAP i.d. 2.0 Base Kit (includes the following components) | |||
|---|---|---|---|
| A | Base Unit (1) | Accepts frames and facilitates immunodetection | SNAP2BASE |
| B | Tubing Assembly Kit (1) | Connects system to vacuum source | |
| C | Blot roller (1) | Eliminates air bubbles between the blot and blot holder (Cat. No. SNAP2RL) | |
| D | Rolling pad (1) | Provides smooth surface for assembling blot | |
| E | Wetting tray (2) | Used for wetting out blot holder and blot | |
| F | Antibody Collection Tray (2) | Collects antibody for recycling (Cat. No. SNAP2ABTR) | |
| G | Quick Start Guide (not shown) | ||
| H | SNAP i.d. 2.0 MultiBlot Holding Frame and Lid | Accepts MultiBlot holders for blot incubation | SNAP2FRMB01 |
| I | SNAP i.d. 2.0 Mini Blot Holding Frame and Lid | Accepts Mini blot holders for blot incubation | SNAP2FRMN01 SNAP2FRMN02 |
| J | SNAP i.d. 2.0 Midi Blot Holding Frame and Lid | Accepts Midi blot holders for blot incubation | SNAP2FRMD01 SNAP2FRMD02 |
| Blot Holders | |||
| K | SNAP i.d. 2.0 MultiBlot Holder (includes 2 MultiBlot well blanks) | Accepts up to 4.5 x 8.4 cm blots Blank is used to block empty chamber when running only one blot |
SNAP2BHMB050 |
| L | SNAP i.d. 2.0 Mini Blot Holder | Accepts up to 7.5 x 8.4 cm blots | SNAP2BHMN0100 |
| M | SNAP i.d. 2.0 Midi Blot Holder | Accepts up to 8.5 x 13.5 cm blots | SNAP2BHMD0100 |
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