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Taq DNA Polymerase
Taq DNA Polymerase
Place of Origin:
China
Brand:
Shanghai Huaguan Biochip Co., Ltd.
Model:
Supplier:
Shanghai Huaguan Biochip Co., Ltd.
Price:
Hits:
2262 
Updated:
4/24/2011
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    Taq DNA Polymerase

    Description

    Taq DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and also possesses a 5´→3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures, but is not recommended for use in DNA sequencing reactions. Our Taq DNA Polymerase is provided in the storage buffer compatible with any reaction buffer designed for Taq DNA polymerase, which containing 20mM Tris-HCl (pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% glycerol, 0.5% Nonidet-P40 and 0.5% Tween 20.

    10X Reaction Buffer without MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C) and 1% Triton X-100. Coupled with separate 25mM MgCl2 Solution.

    10X Reaction Buffer with MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C), 1% Triton X-100 and 15mM MgCl2. Optimized for use with 0.2mM of each dNTP.

    Features

    Reliable: Compositions of the storage buffer have been optimized to assure quality performance of the enzyme under a variety of conditions.

    Flexible: Choose either Taq with 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2.

    Performance Guarantee: Our enzymes and reagents are proven in PCR to ensure reliable, high-performance results.

    Storage Conditions

    Store at –20°C.

    Quality Control Tests

    PCR/activity, SDS-PAGE/purity.

    Please contact us for more information.
    bio-equip.cn
    Shanghai Biochip Co., Ltd.（SBC） was founded in Aug. 2001. The existing shareholders of the company include some well-known universities, research institutes, hospitals and enterprises in China. In Feb. 2003, the National Development and Reform Commission authorized SBC to build up and operate the National Engineering Center for biochip at Shanghai project. The company is located in the Zhangjiang High-tech Park Biotech&Pharmaceutical Industrial Base in Pudong Shanghai with a total area of 40,000 square meters, a construction area of over 20,000 square meters, and a current employee number of more than 300 people.

    In accordance with company's development strategy and the construction requirements raised by National Development and Reform Commission for the National Engineering Center, SBC is committed to applying and promoting biotechnology development and products for life science research、health-care、 drug discovery and other fields. Through more than 10 years of construction and development, the technology innovation service system is established which is based on the core platforms of the biological sample bank, tissue chips, gene expression chips and the next-generation sequencing; The industrialized system which takes molecular medical diagnosis technology and product development as the core competitiveness has been set up; And three main business areas which include the services for research and drugs R&D, molecular medicine inspection services, and production and sales of molecular diagnostic product.

    SBC obeys the value idea of “Scientific innovation, Business orientation, Career development”, follows the construction requirements for the National Engineering Center, and strives to make the company an internationally-advanced national bio-chip industrialization base which integrates the function of technology innovation, achievements transformation, comprehensive service and personal training as a whole. What’s more, the company’s strategic goal is to be an innovative benchmark enterprise among the industry with an level internationally-advanced and has the capability of independent research and development. It would make a contribution to the goal of making Shanghai an influential technology innovation center and also contribute to the domestic field of biomedical science and technology innovation and industrialization in our country.
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