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SureSeq™ FFPE DNA Repair Mix*
SureSeq™ FFPE DNA Repair Mix*
Place of Origin:
United Kingdom
Oxford Gene Technology
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    Repair a broad range of damage in FFPE-derived DNA 

    Detection of single nucleotide variants (SNVs) and insertion/deletions (indels) using next generation sequencing (NGS) technology is gaining increasing importance in research into cancer development and progression. Tissue biopsies are typically archived as formalin-fixed, paraffin-embedded (FFPE) blocks, which preserve tissue morphology and allow long-term storage at room temperature. However, the methods used for fixation significantly damage and compromise the quality of nucleic acids from these samples. Consequently, it may be difficult to distinguish between true and damage-induced low-frequency mutations in such samples. The SureSeq FFPE DNA Repair Mix is a mixture of enzymes that has been optimised to remove a broad range of damage that can cause artefacts in sequencing data (Table 1).

    Table 1

    Table 1: The SureSeq FFPE DNA Repair Mix is capable of removing a variety of DNA damage caused by fixation and long-term storage.

    Improved NGS library yields, on-target rates (%OTR) and mean target coverage

    Obtaining high-quality NGS libraries from FFPE-derived DNA can be a challenging task due to DNA degradation. SureSeq FFPE DNA Repair Mix has been shown to significantly improve NGS library yields (Figure 1), preserving original complexity and delivering high-quality sequencing data for confident calling of variants with low minor allele fractions (MAFs).

    Figure 1

    Figure 1: Example traces from the Agilent® TapeStation illustrating the improvement in pre-capture NGS library yields. Input: 100 ng FFPE DNA.

    One way of increasing NGS test sensitivity is to sequence to very high depths by reducing the number of samples in a run. This approach, however, increases the cost of sequencing reagents per sample processed. By repairing your DNA you can maximise depth of coverage as well as %OTR and therefore increase the sensitivity of your assay without compromising on cost or throughput (Figure 2).

    Figure 2

    Figure 2: The SureSeq FFPE DNA Repair Mix significantly improves mean target coverage resulting in more confident calls. Data obtained using 500 ng of FFPE DNA from ovarian and colon cancer samples; 16 samples per MiSeq® lane.

    More accurate data with lower input DNA

    Pathology labs often have to work with very limited amounts of material. Additionally, FFPE samples are usually irreplaceable. This leads to the need to reduce DNA input in downstream applications including NGS. Often amplicon-based approaches are chosen as they require very little input material. Unfortunately, due to PCR bias and lower complexity from smaller input amounts, these methods are not well suited to detect low-frequency mutations in heterogenous tumour samples. Hybridisation-based approaches eliminate the problem of PCR bias providing much more reliable data but they typically require higher DNA inputs of 500 ng – 1 μg. Using the SureSeq FFPE DNA Repair Mix a reduction in the amount of starting material down to 100 ng depending on required depth of coverage is possible (Figure 3).

    Figure 3

    Figure 3: The SureSeq FFPE DNA Repair Mix allows reduced amount of DNA input by improving mean target coverage. Data obtained using 500, 100 and 50 ng of FFPE DNA.
    Oxford Gene Technology (OGT) provides world-class genetics research solutions to leading clinical and academic research institutions. Founded by Professor Sir Edwin Southern, and with customers in over 60 countries worldwide, OGT has a strong reputation and increasing share in the large and growing genomic medicine market. The Company’s Cytocell®, CytoSure™ and Genefficiency™ range of fluorescence in situ hybridisation (FISH), microarray and next generation sequencing (NGS) products and services deliver high-quality genetic analysis, enabling accurate identification and confirmation of the causative variation underlying genetic disease.
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