For the quantitative determination of Human VD concentrations.
Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 30 nmol/L-1200nmol/L
Components:
| Assay plate (12 × 8 coated Microwells) |
1 |
| Standard:2400 nmol/L |
1×0.5ml |
| Standard Diluent |
1×1.5ml |
| HRP-Conjugate Reagent |
1×6ml |
| Sample Diluent |
1×6ml |
| Chromogen Solution A |
1×6ml |
| Chromogen Solution B |
1×6ml |
| Stop Solution |
1×6ml |
| Wash Solution |
1×20ml×30 fold |
| User manual |
1 |
| Adhesive Strip |
2 |
Product Principle:
The kit is for the quantitative level of VD in the sample, adopt purified Human VD antibody to coat microtiter plate, make
solid-phase antibody, then add VD to wells, Combine VD antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of VD in the samples is then determined by comparing the O.D. of the samples to
the standard curve.
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