EliKine™ Free Thyroxine (fT4) ELISA Kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to fT4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated fT4. A competitive inhibition reaction is launched between fT4 (Standards or samples) and Biotin-conjugated fT4 with the pre-coated antibody specific for fT4. The more amount of fT4 in samples, the less antibody bound by Biotin-conjugated fT4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of fT4 in the sample. The color development is stopped and the intensity of the color is measured.
The EliKine™ Free Thyroxine (fT4) ELISA Kit contains below components:
fT4 microplate
fT4 standard
Biotin conjugated fT4
Avidin-HRP
HRP substrate A
HRP substrate B
Stop solution
Wash buffer
Plate covers.
The Free Thyroxine (fT4) ELISA Kit with the calibration range is: 4 pmol/L-64 pmol/L, and sensitivity is: 4.0 pmol/L.
More Abbkine EliKine™ series of ELISA Kits at EliKine™.
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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.
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