EliKine™ Mouse IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-5 in samples. An antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-5 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The EliKine™ IL-5 Mouse ELISA Kit contains below components:
Mouse IL-5 microplate
Mouse IL-5 standard
Mouse IL-5 detect antibody
EliKine™ Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers.
The Mouse IL-5 ELISA Kit with the calibration range is: 7.8 pg/mL-500 pg/mL, and sensitivity is: 4 pg/mL.
More Abbkine EliKine™ series of ELISA Kits at EliKine™.
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