EliKine™ Human IFN-α ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-α in samples. An antibody specific for IFN-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-α is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-α bound in the initial step. The color development is stopped and the intensity of the color is measured.
The EliKine™ IFN-α Human ELISA Kit contains below components:
Human IFN-α microplate
Human IFN-α standard
Human IFN-α detect antibody
EliKine™ Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers.
More Abbkine EliKine™ series of ELISA Kits at EliKine™.
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