EliKine™ Human HGF ELISA Kit employs a two-site sandwich ELISA to quantitate HGF in samples. An antibody specific for HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
The EliKine™ HGF Human ELISA Kit contains below components:
Human HGF microplate
Human HGF standard
Human HGF detect antibody
EliKine™ Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers.
The Human HGF ELISA Kit with the calibration range is: 125 pg/mL-8000 pg/mL, and sensitivity is: 60 pg/mL.
More Abbkine EliKine™ series of ELISA Kits at EliKine™.
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