EliKine™ Human CRP ELISA Kit employs a two-site sandwich ELISA to quantitate CRP in samples. An antibody specific for CRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRP is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRP bound in the initial step. The color development is stopped and the intensity of the color is measured.
The EliKine™ CRP Human ELISA Kit contains below components:
Human CRP microplate
Human CRP standard
Human CRP detect antibody
Avidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers.
The Human CRP ELISA Kit with the calibration range is: 15.6 pg/mL-1000 pg/mL, and sensitivity is: 8 pg/mL.
More Abbkine EliKine™ series of ELISA Kits at EliKine™.
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