EliKine™ Human CD54 ELISA Kit employs a two-site sandwich ELISA to quantitate CD54 in samples. An antibody specific for CD54 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD54 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD54 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD54 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The EliKine™ CD54 Human ELISA Kit contains below components:
Human CD54 microplate
Human CD54 standard
Human CD54 detect antibody
Avidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers.
More Abbkine EliKine™ series of ELISA Kits at EliKine™.
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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.