EliKine™ Human CCL3 ELISA Kit employs a two-site sandwich ELISA to quantitate CCL3 in samples. An antibody specific for CCL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL3 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The EliKine™ CCL3 Human ELISA Kit contains below components:
Human CCL3 microplate
Human CCL3 standard
Human CCL3 detect antibody
Avidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers.
The Human CCL3 ELISA Kit with the calibration range is: 31.25 pg/mL- 2000 pg/mL, and sensitivity is: 16 pg/mL.
More Abbkine EliKine™ series of ELISA Kits at EliKine™.
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