REAGEN™ Fipronil ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Fipronil in fish, shrimp ,meat , milk ,eggs and honey.
The unique features of the kit are:
- high recovery (80%-105%), and cost-effective extraction methods for various samples.
- High sensitivity (0.025 ng/g or ppb) and low detection limit (0.05 ng/g or ppb) for various samples.
- High reproducibility.
- A quick ELISA assay (less than 1hours regardless of number of samples).
Procedure Overview
REAGEN™The method is based on a competitive colorimetric ELISA assay. The Fipronil antibody has been coated in the plate wells. During the analysis, sample and Fipronil-horseradish peroxidase ( Fipronil-HRP) conjugate are added to the wells for incubation. If the Fipronil residue is present in the sample, it will compete for the Fipronil antibody, thereby preventing the Fipronil-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Fipronil residue concentration in the sample.
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