REAGEN™ Melamine Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect melamine residues in raw commingled cow’s milk and processed milk products. This one-step test requires no sample preparation and is designed for point-of-care use or reference lab settings
The unique features of the kit are:
- Rapid strip test method – 7 minutes
- No sample preparation required
- High sensitivity
- High reproducibility
Mechanism of Reaction
The assay uses a competitive colloidal gold based format. The milk sample (200 μL) is added to a clear plastic strip well or tube and the test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with melamine present in the milk will bind to the melamine imprinted at the Test line (T-line), forming a signal (red line) at that position. If the antibody on the gold particle has engaged with melamine present in the milk sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is stronger than the signal at the C-line indicates a negative result. Signal at the T-line which is equal or less intense compared to the C-line indicates the presence of melamine in the milk. The greater the reduction in signal intensity at the T-line, the greater the concentration of melamine present in the milk.
Sensitivity (Detection Limit)
The sensitivity of the kit in raw commingled cow’s milk is 50ppbusing the RDS-1000 Strip Reader. The limit of detection by visual interpretation is 50-100 ppb.
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