REAGEN™ Fluoroquinolone Strip Test Kit is a qualitative and rapid lateral flow assay designed to detect fluoroquinolone antibiotic residues in raw commingled cow’s milk. This state-of-the-art test uses a broad-spectrum antibody capable of specifically detecting a range of fluoroquinolone residues. This test is designed for point-of-care use or reference lab settings and eliminates the need for a heating block.
The features of the kit are:
Rapid strip test method – 7 minutes
Broad-spectrum antibody that requires no heating step
High sensitivity
High reproducibility
Mechanism of reaction
The assay uses a competitive colloidal gold based format.The milksample(200μL)is added to a clear plastic reaction vessel,and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells.The milk is incubated briefly(3 min)to allow the fluoroquinolone antibody on the gold particles to engage with any fluoroquinolone antibiotic residues present in the milk.The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip.Any gold particles that are not complexed with antibiotics present in the milk will bind to the fluoroquinolone antibiotic imprinted at the Test line(T-line),forming a signal(red line)at that position.If the fluoroquinolone antibody on the gold particle has engaged with antibiotic present in the milk sample,the gold particle will flow past the T-line and reach the Control line(C-line).For visual interpretation of the test results,T-line signal intensity that is stronger than the signal at the C-line indicates a negative result.Signal at the T-line which is equal or less intense compared to the C-line indicates the presence of fluoroquinolone antibiotics in the milk.The greater the reduction in signal intensity at the T-line,the greater the concentration of fluoroquinolone antibiotic residues present in the milk.
Sensitivity (detection limit)
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Fluoroquinolones
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Antibiotic (MRL)
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Detection limit (μg/L)
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Enrofloxacin (100)
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1.5-2.5 ppb
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Ciprofloxacin(100)
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1-2 ppb
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Difloxacin
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20-30 ppb
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Sarafloxacin
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20-30 ppb
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Norfloxacin
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1-2 ppb
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Marbofloxacin (75)
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4-6 ppb
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Danofloxacin (30)
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2-3 ppb
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Flumequine (50)
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4-6 ppb
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