REAGEN™Ceftiofur ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Ceftiofur in milk, urine, tissue (liver, kidney, and muscle), serum, embryonic eggs, and plasma samples..
The features of the kit are:
- High recovery (>80%), low-cost rapid sample prep protocol (10 minutes - 1 hour)
- High sensitivity: 2 ng/gram = 2 ppb (parts per billion)
- Rapid assay protocol (less than 2 hours to process 40 samples in duplicate)
- High reproducibility (interplate and intraplate CV typically <8%)
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The drug of interest (Ceftiofur) has been coated in the plate wells. The sample to be tested is mixed with a high-affinity Ceftiofur capture protein and added to the well. If the Ceftiofur target is present in the sample, it will compete for the capture protein, thereby preventing the capture protein from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the capture protein bound to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. The levels of Ceftiofur in the sample are determined by comparison of the color intensity of unknown samples with the standard curve, which is made using the Ceftiofur standard provided in the kit.
Sensitivity (Detection Limit)
| Sample type |
Detection Limit (ng/g or ppb)
|
|
Milk, Meat, Tissue, Fish and Urine, Embryonic Eggs
|
20
|
|
Serum and Plasma
|
40
|
bio-equip.cn
中文
