Triiodothyronine (T3) is a thyroid hormone that circulates in blood almost completely bound (]99.5%) to carrier proteins. The main transport protein is thyroxine-binding globulin (TBG). Furthermore, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function, as the concentrations of the carrier proteins changes, the total triiodothyronine level also changes, so that the free triiodothyronine concentration remains constant. Measurements of free triiodothyronine (Free T3) concentrations, therefore, correlate more reliably with your clinical status than total triiodothyronine (T3) levels. For example, the increase in total triiodothyronine levels associated with pregnancy, oral contraceptives and estrogen therapy result in higher total T3 levels while the free T3 concentration remains unchanged .
Sheep Free tri-iodothyronine Indes (fT3) ELISA Kit employs a two-site sandwich ELISA to quantitate FT3 in samples. An antibody specific for FT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sheep Free tri-iodothyronine Indes (fT3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/sheep-free-tri-iodothyronine-indes-ft3-elisa-kit-kte110071/
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