Insulin-like Growth Factor I is a member of the insulin superfamily. It was originally discovered as a mediator of growth hormone actions on somatic cell growth, but has also been shown to be an important regulator of cell metabolism, differentiation and survival. IGF-I is synthesized as a preproprotein that is proteolytically cleaved to generate the mature protein linked by three disulfide bonds.Mature IGF-I is highly conserved among mammals, with 100% sequence identity between the Porcine, bovine, porcine, equine and canine proteins. IGF-I is synthesized in the liver and multiple other tissues. It is found in blood and other body fluids as a complex with specific high affinity IGF binding proteins (IGFBP-1to-6).
Sheep Insulin-like growth factor 1 (IGF-1) ELISA Kit employs a two-site sandwich ELISA to quantitate IGF1 in samples. An antibody specific for IGF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIGF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sheep Insulin-like growth factor 1 (IGF-1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/sheep-insulin-like-growth-factor-1-igf-1-elisa-kit-kte110066/
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