Nitric oxide synthase 2A (inducible, hepatocytes)is an enzyme encoded by the NOS2 gene. is produced by a group of enzymes called nitric oxide synthases (NOS). These enzymes catalyze the production of NO and L-citrulline from L-arginine, O2, and NADPH-derived electrons. Mammalian systems contain three well-characterized isoforms of the enzyme: neuronal NOS (nNOS, also called NOS1), inducible NOS (iNOS or NOS2), and endothelial NOS (eNOS or NOS3). The names reflect characteristics of the activity or the original tissues in which the enzymes were first described, but it is now known that each of these isoforms is expressed in a variety of tissues and cell types . The three main isoforms share structural similarities and have nearly identical catalytic mechanisms.
Sheep Nitric oxide synthase 2, inducible (NOS2) ELISA Kit employs a two-site sandwich ELISA to quantitate NOS2 in samples. An antibody specific for NOS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNOS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sheep Nitric oxide synthase 2, inducible (NOS2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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