Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit,Abbkine_specification/price/image

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The main source of ROS is known to be the mitochondria, and increased levels of ROS from the mitochondria have been observed in many cancer cells. Thus far, the mechanism of ROS production in cancer cell proliferation in the mitochondria is not well-understood. Romo1 increased expression of Romo1-triggered ROS production in the mitochondria. The experiments conducted in the present study showed that Romo1-derived ROS were indispensable for the proliferation of both normal and cancer cells. Furthermore, whilst cell growth was inhibited by blocking the ERK pathway in cells transfected with siRNA directed against Romo1, the cell growth was recovered by addition of exogenous hydrogen peroxide. The results of this study suggest that Romo1-induced ROS may play an important role in redox signaling in cancer cells.

Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit employs a two-site sandwich ELISA to quantitate ROMO1 in samples. An antibody specific for ROMO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyROMO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ROMO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ROMO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Reactive oxygen species modulator 1 (ROMO1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/rat-reactive-oxygen-species-modulator-1-romo1-elisa-kit-kte100977/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.