Proliferating Cell Nuclear Antigen a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. It achieves this processivity by encircling the DNA, thus creating a topological link to the genome. It is an example of a DNA clamp. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCNA-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated PCNA-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled PCNA-Ab and unlabeled PCNA-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PCNA-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Rat Proliferating cell nuclear antigen antibody (PCNA-Ab) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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