Gastric inhibitory polypeptide (GIP), is a member of the secretin family of hormones.GIP is derived from a 153-amino acid proprotein encoded by the GIP gene and circulates as a biologically active 42-amino acid peptide. It is synthesized by K cells, which are found in the mucosa of the duodenum and the jejunum of the gastrointestinal tract.Like all endocrine hormones, it is transported by blood. It is now believed that the function of GIP is to induce insulin secretion, which is primarily stimulated by hyperosmolarity of glucose in the duodenum. After this discovery, some researchers prefer the new name of glucose-dependent insulinotropic peptide, while retaining the acronym "GIP." The amount of insulin secreted is greater when glucose is administered orally than intravenously.
Rat Gastric inhibitory polypeptide (GIP) ELISA Kit employs a two-site sandwich ELISA to quantitate GIP in samples. An antibody specific for GIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Gastric inhibitory polypeptide (GIP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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