DCUN1D5 cDNA encoded a protein of 237 amino acids, which contained two domains associated with DNA repair and cell cycle regulation. Although no reported studies have clearly demonstrated DCUN1D5’s role in cancer, one report suggested that DCUN1D5 expression may be useful as a biomarker for cervical cancer. Because DNA damage and repair pathways played critical roles in the development of LSCC, a smokingrelated cancer, and because DCUN1D5 was found to be up-regulated in our patients with LSCC who had a tobacco history, that DCUN1D5 had a vital function involved in DNA damage response and repair in the carcinogenesis of LSCC.
Rat DCN1-like protein 5 (DCUN1D5) ELISA Kit employs a two-site sandwich ELISA to quantitate DCUN1D5 in samples. An antibody specific for DCUN1D5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCUN1D5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCUN1D5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCUN1D5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat DCN1-like protein 5 (DCUN1D5) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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