Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by DGKB is a diacylglycerol kinase, beta isotype. Several alternatively spliced transcript variants encoding different isoforms have been found for DGKB.
Rat Diacylglycerol kinase beta (DGKB) ELISA Kit employs a two-site sandwich ELISA to quantitate DGKB in samples. An antibody specific for DGKB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDGKB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DGKB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DGKB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Diacylglycerol kinase beta (DGKB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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