Serine/threonine-protein kinase N1 encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis.The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit employs a two-site sandwich ELISA to quantitate PKN1 in samples. An antibody specific for PKN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPKN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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