Phospholipase A1 is a phospholipase enzyme which removes the 1-acyl. Phospholipase A1 is a component of snake venom, but it also serves in normal metabolism to produce fatty acids from phospholipids. In melanocytic cells the Phospholipase A1 gene may be regulated by MITF.Phosphatidylserine-specific phospholipase A1-alpha (PLA1A) acts specifically on phosphatidylserine (PS) and 1-acyl-2-lysophosphatidylserine (lyso-PS) to hydrolyze fatty acids at the sn-1 position of these phospholipids.Although PLA1A belongs to the phospholipase gene family, the predicted amino acid sequence of the rat gene product demonstrated greater homology with mammalian triglyceride lipases than with phospholipases.
Rat Phospholipase A1 member A (PLA1A) ELISA Kit employs a two-site sandwich ELISA to quantitate PLA1A in samples. An antibody specific for PLA1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Phospholipase A1 member A (PLA1A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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