Rat Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

uPAR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. All three domains are necessary for high affinity binding of the primary ligand, urokinase. It has been possible to express uPAR recombinantly in CHO-cells and S2 cells. 4 out of 5 of the possible glycosylation sites are used in vivo giving the protein a molecular weight of 50-60 kDA. Recently the structure of uPAR was solved by X-ray crystallography in complex with an peptide antagonist and with its native ligand, urokinase.However the components of the plasminogen activation system have been found to be highly expressed in many malignant tumors, indicating that tumors are able to hijack the system, and use it in metastasis. Thus inhibitors of the various components of the plasminogen activation system has been sought as possible anticancer drugs.

Rat Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit employs a two-site sandwich ELISA to quantitate PLAUR/uPAR in samples. An antibody specific for PLAUR/uPAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLAUR/uPAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAUR/uPAR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAUR/uPAR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Rat Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/rat-urokinase-type-plasminogen-activator-receptor-plaur-upar-elisa-kit-kte100467/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.