PROK2 gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions.
Rat Prokineticin-2 (PROK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROK2 in samples. An antibody specific for PROK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Prokineticin-2 (PROK2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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