PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAsPUS1-1 encodes a protein with an N-terminal mitochondrial leader peptide. Western blot analysis detected full-length PUS1-1 at an apparent molecular mass of 47 kD, and the mature protein, lacking the mitochondrial localization signal, was about 37 kD. The PUS1-2 and PUS1-3 transcripts differ in the 5-prime untranslated region but encode the same protein that begins with an internal methionine (met29) relative to PUS1-1. Immunofluorescence localized PUS1-1 to mitochondria and PUS1-2 and PUS1-3 to nuclei.
Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) ELISA Kit employs a two-site sandwich ELISA to quantitate PUS1 in samples. An antibody specific for PUS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPUS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PUS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PUS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat TRNA pseudouridine synthase A, mitochondrial (PUS1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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