Peripherin, a type III intermediate filament protein, was initially described by Portier et al. (1984) as a cytoskeletal protein present in neurons of the mammalian peripheral nervous system (hence, the name) and in cultured neuroblastoma cells.The amino-terminal region contained potential serine phosphorylation sites. Northern blot analysis detected a corresponding mRNA in ganglia of the peripheral nervous system, including the superior cervical ganglion, ciliary ganglion, and dorsal root ganglion. In the central nervous system, expression was detected in motor nuclei of cranial nerves and ventral horn neurons. Expression was induced by nerve growth factor.
Rat Peripherin (PRPH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPH in samples. An antibody specific for PRPH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Peripherin (PRPH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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