Trypsin-1 is a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.Normally, cationic trypsinogen represents approximately two-thirds of total trypsinogen, while anionic trypsinogen makes up approximately one-third. Mesotrypsinogen is a minor species, accounting for less than 5% of trypsinogens or 0.5% of pancreatic juice proteins.
Rat Protease, Serine 1 (PRSS1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS1 in samples. An antibody specific for PRSS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Protease, Serine 1 (PRSS1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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