Agrin is a large proteoglycan whose best characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. This protein has nine domains homologous to protease inhibitors. It may also have functions in other tissues and during other stages of development.It is major proteoglycan component in glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.This protein binds to several receptors on the surface of skeletal muscle. The receptor which seems to be required for formation of the neuromuscular junction (NMJ) is called the MuSK receptor (Muscle specific kinase).
Rat Agrin (AGRN) ELISA Kit employs a two-site sandwich ELISA to quantitate AGRN in samples. An antibody specific for AGRN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAGRN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGRN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGRN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Agrin (AGRN) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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