The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.Beta adrenergic receptor kinase-2 (ARBK2, BARK-2, G-protein-coupled receptor kinase 3, GRK3) is an intracellular enzyme that phosphorylates G protein-coupled receptors. It was cloned from mice and rats in 1991. The human gene was cloned in 1993.
Rat Beta-adrenergic receptor kinase 2 (ADRBK2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADRBK2 in samples. An antibody specific for ADRBK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADRBK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRBK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRBK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Beta-adrenergic receptor kinase 2 (ADRBK2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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