The receptor is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene.Activation of the adenosine A1 receptor by an agonist causes binding of Gi1/2/3 or Go protein. Binding of Gi1/2/3 causes an inhibition of adenylate cyclase and therefore a decrease in the cAMP concentration.
Rat Adenosine receptor A1 (ADORA1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADORA1 in samples. An antibody specific for ADORA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADORA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADORA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADORA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Adenosine receptor A1 (ADORA1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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