Apoptosis inducing factor is involved in initiating a caspase-independent pathway of apoptosis (positive intrinsic regulator of apoptosis) by causing DNA fragmentation and chromatin condensation. It also acts as an NADH oxidase. Another AIF function is to regulate the permeability of the mitochondrial membrane upon apoptosis.Normally it is found behind the outer membrane of the mitochondria and is therefore secluded from the nucleus. However, when the mitochondria is damaged, it moves to the cytosol and to the nucleus.Inactivation of AIF leads to resistance of embryonic stem cells to death following the withdrawal of growth factors indicating that it is involved in apoptosis.
Rat Apoptosis inducing factor (AIF) ELISA Kit employs a two-site sandwich ELISA to quantitate AIFM1 in samples. An antibody specific for AIFM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAIFM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AIFM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AIFM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Apoptosis inducing factor (AIF) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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