Transfected cells were grown in the presence of a fluorescent fatty acid analog and those that became positive for the dye were sorted and the cDNA recovered. Two classes of clones were identified: one encodes a 699-amino acid protein resembling rat liver fatty acyl CoA synthetase (FACL2), and the second encodes a 646-amino acid protein with 6 potential transmembrane domains. Northern blot analysis showed high levels of FATP in 3T3-L1 adipocytes, with a major 2.9-kb transcript and a minor 3.8-kb form. Expression increased 5-fold during differentiation of preadipocytes to adipocytes. In normal mouse tissues, FATP mRNA levels were highest in skeletal muscle, heart, and fat. Expression studies showed that FATP increased the uptake of labeled oleic acid by 3- to 4-fold, and similar effects were observed with other LCFAs.
Rat Long-chain fatty acid transport protein 1 (SLC27A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC27A1 in samples. An antibody specific for SLC27A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC27A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC27A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC27A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Long-chain fatty acid transport protein 1 (SLC27A1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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