Pancreatic lipase is an enzyme secreted from the pancreas that uses hydrolysis to break apart fat molecules. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat fat droplets. Because the droplets are small, their surface area is greater, allowing the lipase to break apart the fat more effectively. The resulting monomers are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal. Pancreatic lipase is secreted into the duodenum through the duct system of the pancreas. Normally its concentration in serum is very low. Under extreme disruption of pancreatic function, such as pancreatitis or pancreatic adenocarcinoma.
Rabbit Pancreatic triacylglycerol lipase (PNLIP) ELISA Kit employs a two-site sandwich ELISA to quantitate PNLIP in samples. An antibody specific for PNLIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNLIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNLIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNLIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rabbit Pancreatic triacylglycerol lipase (PNLIP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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