Mouse Oxidizided glutathione (GSSG) ELISA Kit,Abbkine_specification/price/image

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Oxidized glutathione represents one percent of the total cellular glutathione (GSH) concentration. Glutathione, the most abundant intracellular non-protein thiol, is formed in cells as a means of protecting against hydroperoxides. As is well known, peroxides are unstable and, upon decomposition, form very deadly free radicals. Glutathione scavenges these free radicals using the thiol bridge (-SH groups), which generates water and yields the oxidized form of glutathione.The appropriate name for glutathione is N(N-y-L-glutamyl-L-cysteinyl-glycine). The easiest way of describing this compound is to envision two identical molecules connected to each other by a thiol bridge. The identical molecules consist of glutamic acid connected through the gamma carbon carboxylic acid terminal to the amino group of the amino acid cysteine, which is connected to the amino group of the amino acid glycine.

Mouse Oxidizided glutathione (GSSG) ELISA Kit employs a two-site sandwich ELISA to quantitate GSSG in samples. An antibody specific for GSSG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGSSG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSSG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSSG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Oxidizided glutathione (GSSG) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-oxidizided-glutathione-gssg-elisa-kit-kte71570/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.