Genes in the mitochondrial respiratory chain complex gene family provide instructions for proteins involved in oxidative phosphorylation, also called the respiratory chain. Oxidative phosphorylation is an important cellular process that uses oxygen and simple sugars to create adenosine triphosphate (ATP), the cell's main energy source. Five protein complexes, made up of several proteins each, are involved in this process. The complexes are named complex I, complex II, complex III, complex IV, and complex V.
Mouse Mitochondrial respiratory chain complex I (MRCC-I) ELISA Kit employs a two-site sandwich ELISA to quantitate MRCC-I in samples. An antibody specific for MRCC-I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRCC-I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRCC-I is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRCC-I bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Mitochondrial respiratory chain complex I (MRCC-I) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-mitochondrial-respiratory-chain-complex-i-mrcc-i-elisa-kit-kte71567/
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