Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). Due to CCL7 possessing two adjacent N-terminal cysteine residues in its mature protein, it is classified among the subfamily of chemokines known as CC chemokines.The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. CCL7 is one of the most pluripotent chemokines, produced by certain tumor cell lines and by macrophages and acting on multiple cell types including monocytes, lymphocytes, eosinophils, basophils, dendritic cells, and natural killer cells.
Mouse Monocyte chemotactic protein 3 (MCP-3) ELISA Kit employs a two-site sandwich ELISA to quantitate CCL7 in samples. An antibody specific for CCL7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Monocyte chemotactic protein 3 (MCP-3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-monocyte-chemotactic-protein-3-mcp-3-elisa-kit-kte71550/
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