Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Mouse GTPase NRas (NRAS) ELISA Kit employs a two-site sandwich ELISA to quantitate NRAS in samples. An antibody specific for NRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRAS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse GTPase NRas (NRAS) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-gtpase-nras-nras-elisa-kit-kte71544/
bio-equip.cn
中文
