Mouse E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit,Abbkine_specification/price/image

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TRIM68 contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene is expressed in many cancer cell lines. Its expression in normal tissues, however, was found to be restricted to prostate. This gene was also found to be differentially expressed in androgen-dependent versus androgen-independent prostate cancer cells.The deduced 485-amino acid protein has a calculated molecular mass of 56 kD. It has a RING finger domain, followed by a B box and a coiled-coil region. The deduced protein contains an N-terminal cys-his cluster, followed by a bipartite nuclear localization signal, a coiled-coil leucine zipper-like domain, and a C-terminal RET finger protein (TRIM27)-like domain.

Mouse E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit employs a two-site sandwich ELISA to quantitate TRIM68 in samples. An antibody specific for TRIM68 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRIM68 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIM68 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIM68 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse E3 ubiquitin-protein ligase TRIM68 (TRIM68) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-e3-ubiquitin-protein-ligase-trim68-trim68-elisa-kit-kte71538/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.