Phosphatidylinositol is a negatively charged phospholipid and a minor component in the cytosolic side of eukaryotic cell membranes.The inositol can be phosphorylated to form phosphatidylinositol phosphate (PIP), phosphatidylinositol bisphosphate (PIP2) and phosphatidylinositol trisphosphate (PIP3). With inositol in the fermentation medium, phosphatidylinositol (PI) content was increased, while phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were decreased. When yeast cells had a higher content of PI, they produced ethanol much more rapidly and tolerated higher concentrations of ethanol. During ethanol shock treatment at 18% (v/v) ethanol, yeast cells with a higher concentration of PI lost their viability much more slowly than those with a lower concentration of PI, indicating that the PI content in these yeast cells can play an important role in ethanol production and ethanol tolerance.
Mouse Phosphatidylinositol antibody IgG/IgM (PI Ab-IgG/IgM) ELISA Kit has been pre-coated with an antibody specific to PI Ab-IgG/IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated PI Ab-IgG/IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled PI Ab-IgG/IgM and unlabeled PI Ab-IgG/IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PI Ab-IgG/IgM in the sample. The color development is stopped and the intensity of the color is measured.
Mouse Phosphatidylinositol antibody IgG/IgM (PI Ab-IgG/IgM) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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