Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit,Abbkine_specification/price/image

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NUBP2 is a member of the NUBP/MRP gene subfamily of ATP-binding proteins. Nakashima et al. (1999) cloned mouse Nubp2 and used the murine sequence as a probe in EST database analysis, followed by screening a human teratoma cDNA library to clone full-length human NUBP2. The 271-amino acid human NUBP2 protein shares 72.6% amino acid similarity with mouse Nubp2. Northern blot analysis detected a 1.4-kb NUBP2 transcript with ubiquitous expression in all human adult and fetal tissues tested, with highest expression in adult skeletal muscle. NUBP2 contains conserved an ATP/GTP binding motif A (P-loop), an ATP/GTP binding motif A-prime, and NUBP/MRP alpha and beta motifs. NUBP2 lacks an additional N-terminal sequence with 4 cysteine residues that is present in NUBP1.

Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit employs a two-site sandwich ELISA to quantitate NUBP2 in samples. An antibody specific for NUBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNUBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NUBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NUBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Cytosolic Fe-S cluster assembly factor NUBP2 (NUBP2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.