The PAX genes, including PAX1, are a highly conserved family of developmental control genes that encode transcription factors and have been shown to play a role in pattern formation during embryogenesis in vertebrates .The deduced 440-residue protein has a calculated molecular mass of 45.7 kD. PAX1 has an extra 70-amino acids 5-prime to the paired box domain compared to mouse Pax1. They noted that PAX1 shows greatest similarity to PAX9.Deutsch et al. (1988) identified a 3.1 kb-Pax 1 transcript during murine embryonic development, whereas no transcripts were detected in adult mouse tissues. Detailed in situ hybridization analysis with frozen embryonic sections demonstrated Pax1 transcripts in the perichordal zone of the developing vertebral column.
Mouse Paired box protein Pax-1 (PAX1) ELISA Kit employs a two-site sandwich ELISA to quantitate PAX1 in samples. An antibody specific for PAX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Paired box protein Pax-1 (PAX1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-paired-box-protein-pax-1-pax1-elisa-kit-kte71457/
bio-equip.cn
中文
